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Sangon Biotech primer 3 software
Primer 3 Software, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+3+software/0+5+primer+software/pm41887375-159-4-10
Average 86 stars, based on 1 article reviews
primer 3 software - by Bioz Stars, 2026-09
86/100 stars

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other:

Article Title: Establishment and identification of six wheat-Thinopyrum ponticum disomic addition lines derived from partial amphiploid Xiaoyan 7430.
Article Snippet: Key message Six wheat-Thinopyrum ponticum disomic addition lines derived from partial amphiploid Xiaoyan 7430 were identified using in situ hybridization and SNP microarray, the homoeologous group and stripe rust resistance of each alien chromosome were determined, and Th. ponticum chromosome-specific markers were developed.. Abstract Xiaoyan 7430 is a significant partial amphiploid, which is used to set up a bridge for transferring valuable genes from Thinopyrum ponticum (Podp.). Barkworth & D.R.

Sequencing:

Article Title: Safflower CtFT genes orchestrating flowering time and flavonoid biosynthesis.
Article Snippet: .. Primers for each CtFT gene sequence were designed with Primer 3 software and then synthesized by Sangon Biotech (ChangChun, China) platform. .. Real-time quantitative PCR was conducted in the Mx3000p Multiplex Quantitative PCR System (Agilent, California, USA) using MonAmpTMChemoHS qPCR Mix purchased from (Monad, Jiangsu, China).

Software:

Article Title: Safflower CtFT genes orchestrating flowering time and flavonoid biosynthesis.
Article Snippet: .. Primers for each CtFT gene sequence were designed with Primer 3 software and then synthesized by Sangon Biotech (ChangChun, China) platform. .. Real-time quantitative PCR was conducted in the Mx3000p Multiplex Quantitative PCR System (Agilent, California, USA) using MonAmpTMChemoHS qPCR Mix purchased from (Monad, Jiangsu, China).

Article Title: Integrated Transcriptome and Proteome Analysis Reveals that the Antimicrobial Griseofulvin Targets<i>Didymella segeticola</i>Beta-Tubulin to Control Tea Leaf Spot
Article Snippet: Because effective control measures are lacking, tea leaf spot caused by Didymella segeticola results in huge tea (Camellia sinensis) production losses on tea plantations in Guizhou Province, southwestern China.. Screening for natural antimicrobial agents with higher control effects against this pathogen and studying their modes of action may contribute to disease management.. Here, Penicillium griseofulvum-derived antimicrobial griseofulvin (GSF) can inhibit the hyphal growth of D. segeticola strain GZSQ-4, with a half-maximal effective concentration of 0.37 μg/ml in vitro and a higher curative efficacy at a lower dose of 25 μg/ml for detached tea twigs.

Article Title: Chronic arsenic exposure induces the time-dependent modulation of inflammation and immunosuppression in spleen
Article Snippet: .. Primers for mouse genes were designed by PRIMER 3 software and synthesized by Sangon Biological Engineering Technology (Shanghai, China) as provided in Table . ..

Article Title: Targeting the IRE1α/JNK-autophagy axis in microglia attenuates retinal neovascularization in oxygen-induced retinopathy.
Article Snippet: RNA concentration and purity were assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific). cDNA was synthesized via reverse transcription using a PrimeScript RT Master Mix Kit. .. Primers were designed with Primer 3 software and synthesized by Sangon Biotech (Shanghai, China) as listed in Table 1. .. Quantitative RT-PCR was conducted on an ABI Prism 7500- HT system using TB Green Premix Ex Taq (TaKaRa Bio Inc, Shiga, Japan)), following MIQE guidelines.

Article Title: Investigation of Linear Amplification Using Abasic Site-Containing Primers Coupled to Routine STR Typing for LT-DNA Analysis
Article Snippet: .. Primer sets were designed using Primer 3 software and were synthesized with or without modification (dSpacer, 6-FAM) by Sangon Biotech, China. ..

Article Title: Fluoride induces immune-inflammatory disorder in the kidneys via histone lysine crotonylation in vivo.
Article Snippet: Total RNA of kidney was isolated from the kidneys with TRIzol reagent (Invitrogen, USA), and first-strand cDNA was synthesized from a 1 μg-aliquot of the total RNA using the oligo-dT primer and reverse transcriptase. .. Primers for genes were designed by PRIMER 3 software and synthesized by Sangon Biological Engineering technology (Shanghai, China)as shown in Table 1. .. Then, PCR amplification was performed using a SYBR Premix ExTaqII kit (Takara, Japan).

Article Title: Investigating the Impact of Estrogen Levels on Voiding Characteristics, Bladder Structure, and Related Proteins in a Mouse Model of Menopause-Induced Lower Urinary Tract Symptoms
Article Snippet: The cDNA was then amplified via PCR using SYBR-Green dye and the ABI Power SYBR-Green PCR Master Mix kit (Applied Biosystems, Foster City, CA, USA). .. Mouse primers were designed using Primer 3 software and synthesized by Sangon Biotech Co., Ltd. (Shanghai, China) ( ). ..

Synthesized:

Article Title: Safflower CtFT genes orchestrating flowering time and flavonoid biosynthesis.
Article Snippet: .. Primers for each CtFT gene sequence were designed with Primer 3 software and then synthesized by Sangon Biotech (ChangChun, China) platform. .. Real-time quantitative PCR was conducted in the Mx3000p Multiplex Quantitative PCR System (Agilent, California, USA) using MonAmpTMChemoHS qPCR Mix purchased from (Monad, Jiangsu, China).

Article Title: Integrated Transcriptome and Proteome Analysis Reveals that the Antimicrobial Griseofulvin Targets<i>Didymella segeticola</i>Beta-Tubulin to Control Tea Leaf Spot
Article Snippet: Because effective control measures are lacking, tea leaf spot caused by Didymella segeticola results in huge tea (Camellia sinensis) production losses on tea plantations in Guizhou Province, southwestern China.. Screening for natural antimicrobial agents with higher control effects against this pathogen and studying their modes of action may contribute to disease management.. Here, Penicillium griseofulvum-derived antimicrobial griseofulvin (GSF) can inhibit the hyphal growth of D. segeticola strain GZSQ-4, with a half-maximal effective concentration of 0.37 μg/ml in vitro and a higher curative efficacy at a lower dose of 25 μg/ml for detached tea twigs.

Article Title: Chronic arsenic exposure induces the time-dependent modulation of inflammation and immunosuppression in spleen
Article Snippet: .. Primers for mouse genes were designed by PRIMER 3 software and synthesized by Sangon Biological Engineering Technology (Shanghai, China) as provided in Table . ..

Article Title: Targeting the IRE1α/JNK-autophagy axis in microglia attenuates retinal neovascularization in oxygen-induced retinopathy.
Article Snippet: RNA concentration and purity were assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific). cDNA was synthesized via reverse transcription using a PrimeScript RT Master Mix Kit. .. Primers were designed with Primer 3 software and synthesized by Sangon Biotech (Shanghai, China) as listed in Table 1. .. Quantitative RT-PCR was conducted on an ABI Prism 7500- HT system using TB Green Premix Ex Taq (TaKaRa Bio Inc, Shiga, Japan)), following MIQE guidelines.

Article Title: Investigation of Linear Amplification Using Abasic Site-Containing Primers Coupled to Routine STR Typing for LT-DNA Analysis
Article Snippet: .. Primer sets were designed using Primer 3 software and were synthesized with or without modification (dSpacer, 6-FAM) by Sangon Biotech, China. ..

Article Title: Fluoride induces immune-inflammatory disorder in the kidneys via histone lysine crotonylation in vivo.
Article Snippet: Total RNA of kidney was isolated from the kidneys with TRIzol reagent (Invitrogen, USA), and first-strand cDNA was synthesized from a 1 μg-aliquot of the total RNA using the oligo-dT primer and reverse transcriptase. .. Primers for genes were designed by PRIMER 3 software and synthesized by Sangon Biological Engineering technology (Shanghai, China)as shown in Table 1. .. Then, PCR amplification was performed using a SYBR Premix ExTaqII kit (Takara, Japan).

Article Title: Investigating the Impact of Estrogen Levels on Voiding Characteristics, Bladder Structure, and Related Proteins in a Mouse Model of Menopause-Induced Lower Urinary Tract Symptoms
Article Snippet: The cDNA was then amplified via PCR using SYBR-Green dye and the ABI Power SYBR-Green PCR Master Mix kit (Applied Biosystems, Foster City, CA, USA). .. Mouse primers were designed using Primer 3 software and synthesized by Sangon Biotech Co., Ltd. (Shanghai, China) ( ). ..

Modification:

Article Title: Investigation of Linear Amplification Using Abasic Site-Containing Primers Coupled to Routine STR Typing for LT-DNA Analysis
Article Snippet: .. Primer sets were designed using Primer 3 software and were synthesized with or without modification (dSpacer, 6-FAM) by Sangon Biotech, China. ..



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